Testing the Efficacy of Two Antibiotics, (Tetracycline and Amoxicillin) on Bacteria Isolated from the Intestine of Wild African Catfish (Clarias Gariepinus Burchell, 1822) from Ajiwa Reservoir, Katsina State, Nigeria

 Life Sciences: an International Journal (LSIJ)

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Paper Title ; Testing the Efficacy of Two Antibiotics, (Tetracycline and Amoxicillin) on Bacteria Isolated from the Intestine of Wild African Catfish (Clarias Gariepinus Burchell, 1822) from Ajiwa Reservoir, Katsina State, Nigeria

ABSTRACT

Antibiotics are powerful medicines that fight certain infections. However, illegal dumping of human and livestock wastes into water bodies has severe consequences, including the development of antibioticresistant bacteria. This study isolated and identified bacteria from the intestine of wild Clarias gariepinus (African catfish) from the Ajiwa reservoir. Nine fish samples (W1-W9) yielded five different bacteria species: Pseudomonas aeruginosa, Escherichia coli, Cytrobacter freundii, Acinetobacter baumannii, and Klebsiella pneumoniae. Antibiotic effectiveness was determined using Kirby-Bauer disc diffusion. All isolated bacteria showed 100% effectiveness to Tetracycline, except one Pseudomonas spp (W3), which showed 13.5% effectiveness. However, all isolates were 100% resistant to Amoxicillin. These results suggest that Tetracycline has minimal interference, while Amoxicillin has abundant resistance. The introduction of multi-antibiotic-resistant bacteria from terrestrial environments poses a significant threat to aquatic ecosystems and human health.

KEYWORDS

Antibiotic resistance, Bacteria isolation, Tetracycline, Amoxicillin, Clarias gariepinus

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